11th International Bone Fluid Flow Workshop
October 13-14, 2010 (Prior to ASBMR meeting)
University of Toronto Hart House,
7 Hart House Circle
Toronto, Ontario, Canada
Objectives:
- To facilitate diverse interdisciplinary collaborations between biologists, engineers, material scientists, computer scientists, and clinicians for better understanding of bone pathology and tissue regeneration.
- To exchange cutting-edge research progress and explore future directions in the field.
The organizing committee
- Liyun Wang, PhD, University of Delaware (Co-Chair, lywang@udel.edu)
- Lidan You, PhD, University of Toronto (Co-Chair, youlidan@mie.utoronto.ca)
- Chris Price, PhD, University of Delaware (cprice@udel.edu)
The scientific committee
- Yi-Xian Qin, Ph.D., Stony Brook University (Yi-Xian.Qin@sunysb.edu) (Chair)
- Steve Cowin, Ph.D., City College of New York (scowin@earthlink.net)
- Henry J. Donahue, Ph.D., Penn State College of Medicine (hdonahue@psu.edu)
- Susannah Fritton, Ph.D., City College of New York (fritton@ccny.cuny.edu)
- X. Edward Guo, PhD., Columbia University (egx1@columbia.edu)
- Christopher Jacobs, Ph.D., Columbia University (crj2111@columbia.edu)
- Jenneke Klein-Nulend, Ph.D., ACTA University of Amsterdam, The Netherlands (j.kleinnulend@vumc.nl)
- Shelly Weinbaum, Ph.D., City College of New York (weinbaum@ccny.cuny.edu)
Local committee
- Lidan You, PhD, University of Toronto (Chair, youlidan@mie.utoronto.ca)
- Saja Al-Dujaili, University of Toronto (s.al.dujaili@utoronto.ca)
- Chao Liu, University of Toronto (chao.liu@alumni.utoronto.ca)
Program
******************October 13, Wednesday***********************
12:30 Registration and Refreshment
1:10 Welcome Remarks
Lidan You, Ph.D., U Toronto
Session 1: Skeletal Biology (Music Room, Hart House)
Chairs: Drs. Jenneke Klein-Nulend and Hank Donahue
1:15 Lynda Bonewald
The Role of Mechanical Loading in Osteocyte Viability
1:45 Jane Aubin
Approaches of isolating and characterizing osteoprogenitors/MSCs
2:15 Morris Manolson
What human mutations have taught us about osteoclastic bone resorption
2:45 William Thompson
Perlecan/HSPG2 Helps Maintain the Pericellular Space of the Lacuno-Canalicular System Surrounding Osteocytic Processes in Murine Cortical Bone
3: 15 Coffee break
Session 2: Cellular physical environment (Music Room, Hart House)
Chairs: Drs. Steve Cowin and Liyun Wang
3:30 Henry Donahue
Adaptation to mechanical load in Cx43 deficient mice
4:00 Susannah Fritton
Effects of Reduced Estrogen Levels on Cortical Bone Microporosity
4:30 Yixian Qin
Enhancing mineralization and osteoblastic activity by fluid flow stimulation
5:00 John Frangos
Dynamic intramedullary pressure stimulates bone remodeling
5:30 Hai Qing
Lack of Osteocyte Perilacunar Remodeling with Unloading
6:30 Reception and Banquet (Faculty Club at 41 Willcocks St.)
Keynote address by
Edward Guo:”Research from Good to Great, a Grapevine Concept”
******************October 14, Thursday************************
8:00 Breakfast & poster set up
Session 2: Cellular physical environment (Continued) (Great Hall, Hart House)
Chairs: Drs. Susannah Fritton and Yixian Qin
8:30 Divya Sharma
Changes in the Interstitial Fluid Pathway Surrounding Osteocytes in Estrogen-Deficient Rats
8:45 Hiroshi Kamioka
Microstructure of Osteocyte Cell Processes and Surrounding Bone Matrix Revealed by Ultra-high Voltage Electron Microscopy Tomography.
9:00 Chris Price
In Situ measurement of bone fluid flow
9:15 Steve Cowin
Ramp Loading in Russian Doll Poroelasticity
9:45 Gaffar Gailani
Flow Through the Cement Line and Its Contribution to the Poroelastic Properties of Cortical Bone
10:00 Grant Goulet
Computational Analysis of Bone Fluid Flow
10:30 Coffee Break
Session 3: Mechanotransduction and cell-cell communication (Great Hall, Hart House)
Chairs: Drs. Ed Guo, Chris Jacobs, Sheldon Weinbaum, and Lidan You
10:45 Fred Pavalko
Fluid shear-induced mechanical signaling through focal adhesions in bone cells
11:15 Danielle Wu
Localized Changes in Cellular Conductance in Response to Quantifiable PicoNewton Level Forces Applied Using the Stokesian Fluid Stimulus Probe (SFSP)
11:45 Astrid Bakker
Role for nNOS in the Response of Osteoblasts to Mechanical Stimulation
12:00 Jean Jiang
Mechanistic Regulation of Cx43-hemichannels in Osteocytes in Response to Fluid Flow Shear Stress
12:30 Lunch Break & Poster Session (2hr)
A list of poster presentation is shown below
2:30 Ed Guo
Voltage-Sensitive Calcium Channels May Differentiate Intercellular Calcium Signaling between Osteocyte and Osteoblast Networks under Fluid Flow
3:00 Chris Jacobs
Cellular mechanisms of mechanosensing in bone
3:30 Jenneke Klein-Nulend
Intercellular Communication between Osteocytes and Stem Cells: Implications for Bone Tissue Engineering
4: 00 Coffee Break
4:15 Alayna Loiselle
Loss of Osteoblast-Osteocyte Communication Delays Fracture Healing in Cx43 Conditional Knockout Mice Indicating the Importance of Cellular Response to Fluid Flow in the Healing Process
4:30 Ronald Kwon
Osteocyte-Independent Mechanotransduction of Interstitial Fluid Flow
4:45 Johnny Zhang
The Role of the Sphingosine-1-Phosphate Signaling Pathway in Osteocyte Mechanotransduction
5:00 Shu Zhang
The Role of Primary Cilium and the Cytoskeleton in the Modulation of Ca2+ Influx
5:15 Award Presentation/Closing Remarks
******************* The List of Poster Presentations *******************
(Thursday, 12:30-2:30pm, Great Hall, Hart House)
Session 1: Skeletal Biology
Minyi Hu et al.
Effects of Dynamic Hydraulic Stimulation on Intramedullary Pressure Magnitudes and Trabecular Bone Alterations
Vincent Baro et al.
Effects of Fluid Flow on Indentation and Sliding Tests of Meniscus
Saja Al-Dujaili et al.
Osteocyte Apoptosis Directly and Indirectly Promotes Osteoclast Formation via Soluble Factors
Wing-Yee Cheung et al.
IL-6 and ICAM-1 Mediate Osteocyte Apoptosis-Induced Osteoclast Precursor Adhesion to Endothelium In Vitro
Session 2: Cellular physical environment
Joanna Kaiser et al.
Calcium fluxes within cortical bone fluid may affect osteocyte mechanosensitivity
Brandon Getsinger et al.
Development and Validation of a Miniature Bending Device for Imaging Loaded Bone
X. Zhou et al.
Simulation on solute convection in a fiber-filled bone canaliculus with LBM and Monte-Carlo method
Dina Badawy et al.
Bio-composite microfluidic platforms for bone cell mechanobiology study
Session 3: Mechanotransduction and cell-cell communication
Monserratt Lopez et al
Characterization of Mechanotransduction in Osteoblasts
Marjoleine Willems et al.
Fluoride Inhibits the Response of Bone Cells to Mechanical Loading
Jason Li et al.
Osteocytes differentially respond to different oscillatory fluid flow conditions
Chao Liu et al.
Effect of Cyclic Hydraulic Pressure on Osteocytes
Shu Xing et al.
The role of mechanically-induced ATP release in intercellular communications among bone cells: mathematical model